Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: The summary results inflammation cytokines level by ELISA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis reduced the expression of IgE in the serum of allergic asthma mice. ( A ) ELISA was used to measure the total IgE content (ng/ml) in the serum of mice. ( B ) ELISA was used to detect the content of OVA-specific IgE (ng/ml) in the serum of mice. All experiments were repeated in triplicate to average (n=10). ## p <0.001 vs. control; ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis regulated the balance of Th1/Th2-related cytokines in BALF and lung tissues. ( A–E ) ELISA was used to detect the contents of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in BALF. ( F–J ) QRT-PCR was used to detect the expression of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in lung tissues. β-actin served as a reference gene. All experiments were repeated in triplicate to average (n=10). # p <0.05, ## p <0.001 vs. control; * p <0.05, ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Control

Journal: bioRxiv

Article Title: Spatiotemporal Atlas of Pro-Inflammatory (NF-κB) and Anti-Inflammatory (STAT6) Signalling Using Reporter Mice during mRNA Vaccination

doi: 10.64898/2026.01.29.702227

Figure Lengend Snippet: Bone marrow–derived dendritic cells were treated with 2.5 µg of modified or unmodified mRNA encapsulated in LNPs, with an equivalent amount of empty LNPs, or with vehicle (water). Representative histograms show the percentage of CD40-CD80-CD86-CCR7, and MHCII positive cells assessed by flow cytometry, and IL-12 concentrations measured by ELISA ± SEM at 24 or 48 h post treatment (n = 9 at 24 h; n = 3 at 48 h). ***p < 0.001 versus vehicle (two-way ANOVA, Dunnett’s test).

Article Snippet: The concentration of IL-12 in supernatants from BM-DCs was determined by ELISA using the Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA kit (R&D Systems, Cat. DY499) following the manufacturer’s instructions.

Techniques: Derivative Assay, Modification, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Spatiotemporal Atlas of Pro-Inflammatory (NF-κB) and Anti-Inflammatory (STAT6) Signalling Using Reporter Mice during mRNA Vaccination

doi: 10.64898/2026.01.29.702227

Figure Lengend Snippet: (A, C) Representative ex vivo bioluminescence images of organs dissected from NFκB -luc2 and STAT6 -luc2 reporter mice at (A) 5 hours or (C) 29 days after treatment. Signals are shown using pseudocolors according to the scale bar. Quantification of organ bioluminescence is shown in the bar graphs, representing mean photon emission (photons/s/cm²/sr) ± SEM (n = 6). *p < 0.05, **p < 0.01, ** p < 0.001 versus LNP (two-way ANOVA, Dunnett’s test).Thym: thymus, LN: inguinal lymph nodes, Dued.: duodenum. (B, D) Cytokine levels in plasma were measured (B) 5 hours after administration or (D) at day 29. Cytokine concentrations are presented as bar graphs ± SEM (n = 3). *p < 0.05, * p < 0.01 versus LNP (one-way ANOVA, Dunnett’s test). (E) Antigen-specific antibodies were measured in plasma at day 29 by ELISA. Bar graphs represent mean absorbance ± SEM (n = 12). ** p < 0.001 versus LNP (two-way ANOVA, Dunnett’s test).

Article Snippet: The concentration of IL-12 in supernatants from BM-DCs was determined by ELISA using the Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA kit (R&D Systems, Cat. DY499) following the manufacturer’s instructions.

Techniques: Ex Vivo, Clinical Proteomics, Enzyme-linked Immunosorbent Assay